研究專長 (Research Specialties)

天然產物 (Natural Products)、生物合成 (Biosynthesis)、酵素學 (Enzymology)

研究概要 (Research Summary)

我們主要關注微生物中的生物活性分子和酶，並利用它們來應對人類健康和環境永續性的挑戰，這包括對其結構、生物合成和生物活性的基本了解。(Our primary interest concerns the bioactive molecules and enzymes in microbes, repurposing them to address challenges in human health and environmental sustainability, which includes a fundamental understanding of their structures, biosynthesis, and bioactivities.)

研究方向 (Research Directions)

(1)天然產物發現：為了發現與人類健康相關的微生物天然產物，我們使用基於質譜的檢測、基於色譜的分離和基於核磁共振的表徵等技術。(Natural products discovery: To discover microbial natural products that are relevant to human health, we use techniques such as mass spectrometry-based detection, chromatography-based isolation and NMR-based characterization.)

(2) 將化學與基因聯繫起來：為了更好地了解自然界如何製造分子，我們對已鑑定的天然產物進行體內和/或體外生物合成途徑重建。(Connecting chemistry back to genes: To better understanding how nature makes molecules, we perform in vivo and/or in vitro biosynthetic pathway reconstitution of identified natural products.)

(3) 受演化啟發的探索：為了對編碼天然產物的蛋白質進行基因組挖掘，我們使用生物資訊工具（如序列相似性網絡和系統發育樹）來建構、視覺化和分析蛋白質序列。(Evolution-inspired exploration: To perform genome mining for proteins encoding natural products, we use bioinformatic tools such as sequence similarity networks and phylogenetic trees to construct, visualize and analyze protein sequences.)

(4) 酵素學：為了研究酵素的結構、底物範圍和活性位點，我們使用 AlphaFold 蛋白質結構預測、體外表徵和定點突變。(Enzymology: To study the structure, substrate scope and active site of enzymes, we use AlphaFold protein structure prediction, in vitro characterization and site-directed mutagenesis.)

專利 (Patents)

1. Tsong-Long Hwang, Yu-Chia Chang, Kuei-Hung Lai. 9,11-secosteroids and the use of 9,11-secosteroids in preparing pharmaceutical compositions for inhibiting inflammation. US 12,274,704 B2; Date of patent: Apr. 15, 2025.
2. 張芳榮、吳志中、陳炳宏、黃聰龍、王士維、蒲克仁、蔡逸宏。馬卡多種生物功能解碼。中華民國發明第I 874771號；專利權期間：20250301-20420516。
   Fang-Rong Chang, Chih-Chung Wu, Bing-Hung Chen, Tsong-Long Hwang, Shih-Wei Wang, Ke-Ren Pu, Yi-Hong Tsai. Maca Multiple Biological Functions Decoding. R.O.C. Patent No. I 874771; Patent Period: 20250301-20420516.
3. 黃聰龍、陳俞利、張祐嘉。一種草藥萃取物用於製備抑制一個體感染新型冠狀病毒的醫藥組合物之用途。中華民國發明第I 865864號；專利權期間：20241211-20420406。
   Tsong-Long Hwang, Yu-Li Chen, Yu-Chia Chang. Use of a Herbal Extract for Preparing a Pharmaceutical Composition for Inhibiting Novel Coronavirus Infection in an Individual. R.O.C. Patent No. I 865864; Patent Period: 20241211-20420406.
4. 張芳榮、吳志中、陳炳宏、黃聰龍、王士維、蒲克仁、蔡逸宏。マカ抽出物及其用途(馬卡多種生物功能解碼)。日本特許證特許第7446003號；專利權期間：20240229 -20420503。
   Fang-Rong Chang, Chih-Chung Wu, Bing-Hung Chen, Tsong-Long Hwang, Shih-Wei Wang, Ke-Ren Pu, Yi-Hong Tsai. Maca Extract and Its Uses (Maca Multiple Biological Functions Decoding). Japanese Patent No. 7446003; Patent Period: 20240229 -20420503.
5. Tsong-Long Hwang, Ya-Xuan Wang. Methods for the treatment of diseases associated with activation of inflammasomes. US 12,023,329 B2; Date of patent: Jul. 2, 2024.
6. 謝珮文、黃聰龍、黃鈺凌。基尖葉野牡丹根的水萃取物在對抗冠狀病毒感染上的用途。中華民國發明第I 846385號；專利權期間：20240621 -20430315。
   Pei-Wen Hsieh, Tsong-Long Hwang, Yu-Ling Huang. Use of Aqueous Extract of *Medinilla formosana* Roots for Combating Coronavirus Infection. R.O.C. Patent No. I 846385; Patent Period: 20240621 -20430315.
7. 黃聰龍、汪依璿、張祐嘉、宋秉鈞、陳毓昕。一種第I型甲醯胜肽受體拮抗劑及其用途。中華民國發明第I 843149號；專利權期間：20240521 -20420713。
   Tsong-Long Hwang, Yi-Hsuan Wang, Yu-Chia Chang, Ping-Chun Sung, Yu-Hsin Chen. A Type I Formyl Peptide Receptor Antagonist and Its Uses. R.O.C. Patent No. I 843149; Patent Period: 20240521 -20420713.
8. 廖聰龍、黃聰龍、張祐嘉。一種菊花萃取物及其用於製備抑制發炎反應和促進傷口癒合的醫藥組合物之用途。中華民國發明第I 842129號；專利權期間：20240511 -20421013。
   Tsong-Long Liao, Tsong-Long Hwang, Yu-Chia Chang. A Chrysanthemum Extract and Its Use for Preparing a Pharmaceutical Composition for Inhibiting Inflammation and Promoting Wound Healing. R.O.C. Patent No. I 842129; Patent Period: 20240511 -20421013.
9. 黃聰龍、王雅萱。一種酪氨酸激酶抑製劑在製造用以治療因炎性體激活所致相關疾病的藥物中的用途。中華人民共和國ZL 2020 1 0872049.1；專利權期間：20231208 -20400826。
   Tsong-Long Hwang, Ya-Xuan Wang. Use of a Tyrosine Kinase Inhibitor in the Manufacture of a Medicament for Treating Diseases Associated with Inflammasome Activation. P.R.C. Patent No. ZL 2020 1 0872049.1; Patent Period: 20231208 -20400826.
10. Tsong-Long Hwang, Yu-Li Chen, Yu-Chia Chang. Method for inhibiting infection of SARS-CoV-2 in a subject. US 11,839,610 B2; Date of patent: Dec. 12, 2023.
11. 黃聰龍、陳俞利、柯麥可、薩夫拉托娃瑪賽拉、霍斯塔科娃安娜。一種化合物用於製備抑制新型冠狀病毒感染一個體的醫藥組合物之用途。中華民國發明第I 822075號；專利權期間：20231111 -20420531。
    Tsong-Long Hwang, Yu-Li Chen, Michael Korinek, Marcela Saffranova, Anna Khvastakova. Use of a Compound for Preparing a Pharmaceutical Composition for Inhibiting Novel Coronavirus Infection in an Individual. R.O.C. Patent No. I 822075; Patent Period: 20231111 -20420531.
12. Fang-Rong Chang, Chin-Chung Wu, Bing-Hung Chen, Tsong-Long Hwang, Shih-Wei Wang, Arif Purnomo, Yi-Hong Tsai. Maca extract and use thereof. US 11,771,729 B2; Date of patent: Oct. 3, 2023.
13. Tsong-Long Hwang, Yeh-Long Chen, Cherng-Chyi Tzeng, Chih-Hua Tseng. Neutrophil inflammation inhibitor and uses thereof. EP3876933; Date of patent: Sep. 13, 2023.
14. 黃聰龍、陳義龍、曾誠齊、曾志華。嗜中性白血球發炎抑制劑及其用途。中華人民共和國ZL 2018 8 0099319.0；專利權期間：20230714 -20381109。
    Tsong-Long Hwang, Yeh-Long Chen, Cherng-Chyi Tzeng, Chih-Hua Tseng. Neutrophil Inflammation Inhibitor and Its Uses. P.R.C. Patent No. ZL 2018 8 0099319.0; Patent Period: 20230714 -20381109.
15. 黃聰龍、高定一、吳天賞。好中球の活性化及び動員の異常調節に関連する疾患の治療方法 (用以治療與嗜中性白血球激活失調及趨化相關之疾病的方法)。日本特許證特許第7244121號；專利權期間：20230313 -20410423。
    Tsong-Long Hwang, Ding-Yi Kao, Tian-Shang Wu. Methods for Treating Diseases Associated with Dysregulated Neutrophil Activation and Chemotaxis. Japanese Patent No. 7244121; Patent Period: 20230313 -20410423.
16. 黃聰龍、陳義龍、曾誠齊、曾志華。好中球性炎症抑制剤及びその使用(嗜中性白血球發炎抑制劑及其用途。日本特許證特許第7205840號；專利權期間：20230106 -20381109。
    Tsong-Long Hwang, Yeh-Long Chen, Cherng-Chyi Tzeng, Chih-Hua Tseng. Neutrophil Inflammatory Inhibitor and Its Uses. Japanese Patent No. 7205840; Patent Period: 20230106 -20381109.
17. 黃聰龍、姜智釗、賴奎宏、汪依璿、張祐嘉。一種中草藥組合物用於製備預防或治療皮膚病癥的外用藥物的用途。中華民國發明第I 786424號；專利權期間：20221211 -20400806。
    Tsong-Long Hwang, Chih-Chao Chiang, Kuei-Hung Lai, Yi-Hsuan Wang, Yu-Chia Chang. Use of a Chinese Herbal Composition for Preparing Topical Medicaments for Preventing or Treating Skin Conditions. R.O.C. Patent No. I 786424; Patent Period: 20221211 -20400806.
18. 黃聰龍、高定一、吳天賞。用以治療與嗜中性白血球激活失調及趨化相關之疾病的方法。中華民國發明第I 775408號；專利權期間：20220821 -20410425。
    Tsong-Long Hwang, Ding-Yi Kao, Tian-Shang Wu. Methods for Treating Diseases Associated with Dysregulated Neutrophil Activation and Chemotaxis. R.O.C. Patent No. I 775408; Patent Period: 20220821 -20410425.
19. 黃聰龍、王雅萱。用以治療因發炎體激活所致相關疾病的方法。中華民國發мі I 769506號；專利權期間：20220701 -20400825。
    Tsong-Long Hwang, Ya-Xuan Wang. Methods for Treating Diseases Associated with Inflammasome Activation. R.O.C. Patent No. I 769506; Patent Period: 20220701 -20400825.
20. 黃聰龍、陳柏任。利博西林用於製備治療罹患磷酸二酯酶4介導疾病的患者的醫藥組合物及用途。中華民國發明第I 769382號；專利權期間：20220701 -20390620。
    Tsong-Long Hwang, Po-Jen Chen. Ribociclib for Preparing a Pharmaceutical Composition and Its Use for Treating Patients Suffering from Phosphodiesterase 4-Mediated Diseases. R.O.C. Patent No. I 769382; Patent Period: 20220701 -20390620.
21. 黃聰龍、陳柏任、曾馨慧。帕博西林用於製備治療罹患疾病的患者及抑制磷脂醯肌醇3-激酶的活性的醫藥組合物及用途。中華人民共和國ZL 2020 1 0078609.6；專利權期間：20220624 -20400203。
    Tsong-Long Hwang, Po-Jen Chen, Hsin-Hui Tseng. Palbociclib for Preparing a Pharmaceutical Composition and Its Use for Treating Patients Suffering from Diseases and Inhibiting Phosphatidylinositol 3-Kinase Activity. P.R.C. Patent No. ZL 2020 1 0078609.6; Patent Period: 20220624 -20400203.
22. Tsong-Long Hwang, Po-Jen Chen. Pharmaceutical composition and use for applying ribociclib in phosphodiesterase 4-mediated disease treatment of patient and inhibition of phosphodiesterase 4 activity. US 11,351,172 B2; Date of patent: Jun. 7, 2022.
23. 黃聰龍、陳柏任。利博西林用於製備治療罹患磷酸二酯酶4介導疾病的患者的醫藥組合物及用途。中華人民共和國ZL 2020 1 0078474.3；專利權期間：20220419 -20400203。
    Tsong-Long Hwang, Po-Jen Chen. Ribociclib for Preparing a Pharmaceutical Composition and Its Use for Treating Patients Suffering from Phosphodiesterase 4-Mediated Diseases. P.R.C. Patent No. ZL 2020 1 0078474.3; Patent Period: 20220419 -20400203.
24. 黃聰龍、張祐嘉、賴奎宏。9,11-開環類固醇 (9,11-secosteroids) 類化合物及其用於製備抑制發炎之醫藥組合物的用途。中華民國發明第I 742793號；專利權期間：20211011 -20400806。
    Tsong-Long Hwang, Yu-Chia Chang, Kuei-Hung Lai. 9,11-Secosteroids and Their Use for Preparing Pharmaceutical Compositions for Inhibiting Inflammation. R.O.C. Patent No. I 742793; Patent Period: 20211011 -20400806.
25. 黃聰龍、陳柏任、曾馨慧。帕博西林用於製備治療罹患疾病的患者的醫藥組合物的用途。中華民國發明第I 737999號；專利權期間：20210901 -20390528。
    Tsong-Long Hwang, Po-Jen Chen, Hsin-Hui Tseng. Palbociclib for Preparing a Pharmaceutical Composition and Its Use for Treating Patients Suffering from Diseases. R.O.C. Patent No. I 737999; Patent Period: 20210901 -20390528.
26. 黃聰龍、蔡永豐、陳俊宇、郭亮鉾、鄭源斌、王至欣、張芳榮、吳永昌。第I型甲醯胜肽受體拮抗劑及其用途。中華人民共和國ZL 2016 1 1257556.4；專利權期間：20210326 -20361229。
    Tsong-Long Hwang, Yung-Fong Tsai, Chun-Yu Chen, Liang-Mou Kuo, Yuan-Bin Cheng, Chih-Hsin Wang, Fang-Rong Chang, Yang-Chang Wu. Type I Formyl Peptide Receptor Antagonist and Its Uses. P.R.C. Patent No. ZL 2016 1 1257556.4; Patent Period: 20210326 -20361229.
27. 王瑞瑜、黃聰龍、呂彥禮、方嘉佑、吳佩玲、陳瑋琪、柯錇錡。一種滋潤皮膚、具有良好的抗發炎效果之組成物。中華民國發明第I 718407號；專利權期間：20210211 -20380910。
    Ruey-Yu Wang, Tsong-Long Hwang, Yann-Lii Leu, Jia-You Fang, Pei-Ling Wu, Wei-Chi Chen, Pei-Chi Ko. A Composition for Moisturizing Skin and Having Good Anti-Inflammatory Effects. R.O.C. Patent No. I 718407; Patent Period: 20210211 -20380910.
28. Tsong-Long Hwang, Yeh-Long Chen, Cherng-Chyi Tzeng, Chih-Hua Tseng. Neutrophil inflammation inhibitor and uses thereof. US 10,654,848 B1; Date of patent: May 19, 2020.
29. 黃聰龍、陳義龍、曾誠齊、曾志華。嗜中性白血球發炎抑制劑及其用途。中華民國發明第I 680973號；專利權期間：20200101 -20381108。
    Tsong-Long Hwang, Yeh-Long Chen, Cherng-Chyi Tzeng, Chih-Hua Tseng. Neutrophil Inflammation Inhibitor and Its Uses. R.O.C. Patent No. I 680973; Patent Period: 20200101 -20381108.
30. 陳日榮、簡麗娥、黃聰龍、鄭凱元。化合物及其醫藥組合物、用途及萃取自枇杷葉之方法。中華民國發明第I 640505號；專利權期間：20181111 -20361219。
    Jih-Rong Chen, Li-O Jan, Tsong-Long Hwang, Kai-Yuan Cheng. Compounds, Pharmaceutical Compositions Thereof, Uses, and Methods for Extracting from Loquat Leaves. R.O.C. Patent No. I 640505; Patent Period: 20181111 -20361219.
31. Tsong-Long Hwang, Yung-Fong Tsai, Chun-Yu Chen, Liang-Mou Kuo, Yuan-Bin Cheng, Chih-Hsin Wang, Fang-Rong Chang, Yang-Chang Wu. Formyl peptide receptor 1 antagonists and uses thereof. US 9,895,329 B1; Date of patent: Feb. 20, 2018.
32. 黃聰龍、謝珮文、黃吟婷、洪志豪。FPR1拮抗劑的衍生物及其用途(一)。中華民國發明第I 614021號；專利權期間：20180211 -20331008。
    Tsong-Long Hwang, Pei-Wen Hsieh, Yin-Ting Huang, Chih-Hao Hung. FPR1 Antagonist Derivatives and Uses Thereof (I). R.O.C. Patent No. I 614021; Patent Period: 20180211 -20331008.
33. 黃聰龍、蔡永豐、陳俊宇、郭亮鉾、鄭源斌、王至欣、張芳榮、吳永昌。第I型甲醯胜肽受體拮抗劑及其用途。中華人民共和國ZL 2016 1 1257556.4；專利權期間：20171201 -20361228。
    Tsong-Long Hwang, Yung-Fong Tsai, Chun-Yu Chen, Liang-Mou Kuo, Yuan-Bin Cheng, Chih-Hsin Wang, Fang-Rong Chang, Yang-Chang Wu. Type I Formyl Peptide Receptor Antagonist and Its Uses. P.R.C. Patent No. ZL 2016 1 1257556.4; Patent Period: 20171201 -20361228.
34. 謝珮文、黃聰龍、王雯慧、王婷儀。2-胺基苯甲醛肟衍生物及其製備方法與用途。中華人民共和國ZL 2014 1 0199178.3；專利權期間：20170412 -20340511。
    Pei-Wen Hsieh, Tsong-Long Hwang, Wen-Hui Wang, Ting-Yi Wang. 2-Aminobenzaldehyde Oxime Derivatives, Their Preparation Methods and Uses. P.R.C. Patent No. ZL 2014 1 0199178.3; Patent Period: 20170412 -20340511.
35. Tsong-Long Hwang, Pei-Wen Hsieh, Yin-Ting Huang, Chih-Hao Hung. FPR1 antagonist derivatives and use thereof. US 9,593,144 B2; Date of patent: Mar. 14, 2017.
36. 陳金銓、呂彥禮、黃聰龍、方嘉佑、潘台龍。哺乳類SIRT1的活化劑。中華民國發明第I 556818號；專利權期間：20161111 -20350806。
    Chin-Chuan Chen, Yann-Lii Leu, Tsong-Long Hwang, Jia-You Fang, Tai-Lung Pan. Mammalian SIRT1 Activators. R.O.C. Patent No. I 556818; Patent Period: 20161111 -20350806.
37. 黃聰龍、謝珮文、黃吟婷、洪志豪。FPR1拮抗劑的衍生物及其用途。中華民國發明第I 537251號；專利權期間：20160611 -20331008。
    Tsong-Long Hwang, Pei-Wen Hsieh, Yin-Ting Huang, Chih-Hao Hung. FPR1 Antagonist Derivatives and Uses Thereof. R.O.C. Patent No. I 537251; Patent Period: 20160611 -20331008.
38. Pei-Wen Hsieh, Tsong-Long Hwang, Wen-Hui Wang, Ting-Yi Wang. Oxime-based compound, pharmaceutical composition containing the same and method for preparing the same. US 9,073,833 B1; Date of patent: Jul. 7, 2015.
39. 黃聰龍、謝珮文、余黃平。苯并雜氧嗪酮衍生物，其製備方法以及包含有此等衍生物的藥學組成物。中華民國發明第I 419884號；專利權期間：20131221 -20300623。
    Tsong-Long Hwang, Pei-Wen Hsieh, Huang-Ping Yu. Benzoxazinone Derivatives, Their Preparation Methods, and Pharmaceutical Compositions Containing Such Derivatives. R.O.C. Patent No. I 419884; Patent Period: 20131221 -20300623.
40. 宋秉鈞、白鎮豪、蘇尹帝、黃聰龍、李展榮、林美如、許志宏、方力行。白鞭珊瑚酯及其分離方法。中華民國發明第I 353355號；專利權期間：20111201 -20280611。
    Ping-Chun Sung, Chen-Hao Pai, Yin-Ti Su, Tsong-Long Hwang, Chan-Jung Li, Mei-Ru Lin, Chih-Hung Hsu, Li-Hsing Fang. Pseudopterogorgia elisabethae Esters and Their Isolation Methods. R.O.C. Patent No. I 353355; Patent Period: 20111201 -20280611.
41. 宋秉鈞、白鎮豪、蘇尹帝、黃聰龍、郭富雯、樊同雲、李展榮。紅鞭珊瑚醇及其分離方法。中華民國發明第I 344960號；專利權期間：20110711-20280611。
    Ping-Chun Sung, Chen-Hao Pai, Yin-Ti Su, Tsong-Long Hwang, Fu-Wen Kuo, Tung-Yun Fan, Chan-Jung Li. *Gorgonia* Alcohols and Their Isolation Methods. R.O.C. Patent No. I 344960; Patent Period: 20110711-20280611.

五年內著作目錄 (Selected Papers)

1. Chen SH, Chen CH, Lin HC, Yeh SA, Hwang TL*, Chen PJ*. Drug repurposing of cyclin-dependent kinase inhibitors for neutrophilic acute respiratory distress syndrome and psoriasis. J Adv Res. 2025 Jun:72:485-500.
2. Chiang CC, Cheng, WJ, Dela Cruz JRMS, Raviraj T, Wu NL*, Korinek M*, Hwang TL*. Neutrophils in Atopic Dermatitis. Clin Rev Allergy Immunol. 2024 Dec;67(1-3):21-39. Epub 2024 Sep 18.
3. Chen PJ, Chen SH, Chen YL, Wang YH, Lin CY, Chen CH, Tsai YF, Hwang TL*. Ribociclib leverages phosphodiesterase 4 inhibition in the treatment of neutrophilic inflammation and acute respiratory distress syndrome. J Adv Res. 2024 Aug:62:229-243. Epub 2024 Mar 27.
4. Chen PJ, Tseng HH, Wang YH, Fang SY, Chen SH, Chen CH, Tsai SC, Chang YC, Tsai YF, Hwang TL*. Palbociclib blocks neutrophilic phosphatidylinositol 3-kinase activity to alleviate psoriasiform dermatitis. Br J Pharmacol. 2023 Aug;180(16):2172-2188. Epub 2023 Apr 19.
5. Yang SC, Wang YH, Ho CM, Tsai YF, Sung PJ, Lin TE, Hwang TL*. Targeting formyl peptide receptor 1 with anteiso-C13-surfactin for neutrophil-dominant acute respiratory distress syndrome. Br J Pharmacol. 2023 Aug;180(16):2120-2139. Epub 2023 Apr 10.

實驗室活動剪影 (Laboratory Activities Snapshots)